Chloroquine autophagy mechanism

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  1. antonme New Member

    Chloroquine autophagy mechanism


    Pharmacological modulators of this pathway have been extensively utilized in a wide range of basic research and pre-clinical studies. Bafilomycin A1 and chloroquine are commonly used compounds that inhibit autophagy by targeting the lysosomes but through distinct mechanisms.

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    Abstract. Objective We attempt to investigate the mechanism triggered by Chloroquine CQ and its association with autophagic function. Methods To investigate the CQ effects on non-malignant and malignant cells, we performed cell viability assays, flow cytometry analysis, immunoblotting, immunofluorescence, confocal microscopy, electronic microscopy and Fluorescence Lifetime Imaging. Pharmacological modulators of this pathway have been extensively utilized in a wide range of basic research and pre-clinical studies. Bafilomycin A1 and chloroquine are commonly used compounds that inhibit autophagy by targeting the lysosomes but through distinct mechanisms. Jul 11, 2018 In the present study, the cytotoxic mechanism underlying the autophagy inhibitor CQ in cholangiocarcinoma QBC939 cells was elucidated through in vitro experiments. ER stress and autophagy inhibition occur during the stimulation of cells by CQ, resulting in intrinsic, extrinsic and autophagy-dependent apoptosis.

    To address this, we cultured primary rat cortical neurons from E18 embryos and used the Seahorse XF96 analyzer and a targeted metabolomics approach to measure the effects of bafilomycin A1 and chloroquine on bioenergetics and metabolism. Since it is now clear that mitochondrial quality control, particularly in neurons, is dependent on autophagy, it is important to determine whether these compounds modify cellular bioenergetics.

    Chloroquine autophagy mechanism

    Chloroquine Targets Pancreatic Cancer Stem Cells via., Inhibition of autophagy with bafilomycin and chloroquine.

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  4. Recently, an altered chloroquine-transporter protein CG2 of the parasite has been related to chloroquine resistance, but other mechanisms of resistance also appear to be involved. Research on the mechanism of chloroquine and how the parasite has acquired chloroquine resistance is still ongoing, as other mechanisms of resistance are likely.

    • Chloroquine - Wikipedia.
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    Autophagy is a self-digesting mechanism responsible for removal of damaged organelles, malformed proteins during biosynthesis, and nonfunctional long-lived proteins by lysosome. Autophagy has been divided into three general types depending on the mechanism by which intracellular materials are delivered into lysosome for degradation that is, microautophagy, chaperone-mediated autophagy CMA. In a lot of ways, autophagy is the natural process that does all three of these things for your body. The term "autophagy" comes from the Latin word that means "self-eating." This is because the process of autophagy breaks down parts of your cells in order to recycle them in the creation of new cells. Vival, both in vitro and in vivo. Additionally, the autophagy inhibitor chloroquine works in an autophagy-independent manner, and exocytosis may be a mechanism for chloro-quine resistance in AML cells. These findings will have a significant impact on autophagy- and chloroquine-related leukemia therapy and drug discovery. Results

     
  5. idler Moderator

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  8. Vald76 Guest

    P62/Sequestosome-1 Up-regulation Promotes ABT-263-induced. In addition to genetic means, autophagy can be disabled by the lysosomal inhibitor chloroquine or by bortezomib, which blocks autophagy via a cathepsin-dependent mechanism resulting in up-regulation of p62. p62 is rich in protein-interacting sequences and contains domains that include a protein-protein interaction module PB1 that facilitates its oligomerization, a ubiquitin-associated UBA 3 domain, and an LC3 interaction region that are essential for p62 function 8, 13, 14.

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