Chloroquine phoenix cells

Discussion in 'Canadian Drugs' started by Happyginger, 28-Feb-2020.

  1. Gamer User

    Chloroquine phoenix cells


    The lines are based on the 293T cell line (a human embryonic kidney line transformed with adenovirus E1a and carrying a temperature sensitive T antigen co-selected with neomycin). The unique feature of this cell line is that it is highly transfectable with either calcium phosphate mediated transfection or lipid-based transfection protocols-- up to 50% or higher of cells can be transiently transfected.

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    Phoenix is a second-generation retrovirus producer lines for the generation of helper free ecotropic and amphotropic retroviruses. The lines are based on the 293T cell line a human embryonic kidney line transformed with adenovirus E1a and carrying a temperature sensitive T antigen co-selected with neomycin. Chloroquine is a 4-aminoquinoline with antimalarial, anti-inflammatory, and potential chemosensitization and radiosensitization activities. Although the mechanism is not well understood, chloroquine is shown to inhibit the parasitic enzyme heme polymerase that converts the toxic heme into non-toxic hemazoin, thereby resulting in the accumulation of toxic heme within the parasite. Chloroquine is the generic form of the brand-name prescription medicine Aralen, which is used to prevent and treat malaria — a mosquito-borne disease caused by a parasite — and to treat.

    The lines offered advantages over previous stable systems in that virus can be produced in just a few days. The lines were created by placing into 293T cells constructs capable of producing gag-pol, and envelope protein for ecotropic and amphotropic viruses.

    Chloroquine phoenix cells

    Hydroxychloroquine / Plaquenil and the Covid-19 / Coronavirus., Chloroquine C18H26ClN3 - PubChem

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  6. Phoenix cells were incubated in 25 μM chloroquine 5 min prior to transfection with 10 μg retroviral plasmid DNA by the calcium phosphate technique. At 72 h post-transfection, the virus-containing medium was collected and the virus titre determined using NIH3T3 cells.

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    Chloroquine-treated ARPE-19 cells demonstrate a marked increase in vacuolation and dense intracellular debris. These are identified as chloroquine-dilated lysosomes and lipid bodies with LAMP-2 and LipidTOX co-localization, respectively. Dilation is an indicator of lysosomal dysfunction. Lipo2000 is a general transfection reagent that works on many different types of cells. Is it suspension cell type? if so it will be more difficult to transfect. Let us know your finding and good luck! The theory of the experiment with primate cells was that chloroquine could be for preventing viral infection or as a treatment for viral infection after it had occurred. In vitro in these primate.

     
  7. lennick User

    We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising. Inhibition of autophagy as a new means of improving. Chloroquine in Cancer Therapy A Double-Edged Sword of. What does increasing both LC3 II and p62 mean?
     
  8. BarBariS New Member

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